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There is a more than 1,000 year history of semen freezing in horses. It was used in the Eastern states of the U.S. about the turn of the 20th century and became popular when European horses were being imported into North America during the 1920s and 1930s. In the 1940s, the first commercial freezing system, developed by Dr. Bernard Travison, a Belgian veterinarian, was used to make frozen semen available for artificial insemination (AI). The first report of the use of frozen semen in horses was published in 1990, and the first commercial semen freezer was installed in the U.S. in 1999.
The use of frozen semen has not only been tested in horses, but also in dogs, cows, pigs and buffalo. The U.S. Department of Agriculture (USDA) estimates that about 20% of the American dairy herd is comprised of heifers with frozen semen. Due to the increased care and expense associated with breeding, frozen semen is not available for stud breeding for all breeds of dairy cattle. It would be a valuable supplement for assisted reproduction in dairy breeds, which generally have very low reproductive efficiency.
The freezing of semen has been used to improve the reproductive performance of inseminated females. The process of preparing semen for freezing alters the spermatozoa, including removing motile spermatozoa and washing the remaining semen. Motility is lost in frozen semen, but it can be restored to some degree. Motility is usually regained in frozen semen after thawing, but the ability to fertilize will depend on how the semen was frozen. By freezing semen, the viability of spermatozoa and the overall sperm quality is generally maintained. In addition, the freezing process appears to minimize the effects of microorganisms on semen, which may be detrimental to the fertility of the spermatozoa. Frozen semen has been successfully used as a supplement for reproductive assistance in horses, but it has not been well studied in other species of livestock.
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