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ODN 2088 treatment caused a slight reduction of activated microglia in bin 1, and the number of microglia in bin 2 was significantly increased compared to plasmid DNA-treated brains (Fig. 5C). In the control (intact) and ODN 2088-treated brains, the number of microglia in bin 1 was significantly smaller than that of the plasmid DNA-treated brains, but there was no significant difference between the plasmid DNA + ODN 2088-treated and control brains. As noted in the previous page, ODN 2088 treatment, if we used endotoxin-free plasmid DNAs, improved much more than using endotoxin-containing ones (Fig. 7), suggesting that LPS contamination in plasmid DNA solution might be the cause that ODN 2088 treatment could not completely restore microglial aberrant distribution (Fig. 5).
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