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Matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-MSI) allows us to investigate the distribution of lipid molecules within tissues. We used MALDI-MSI to identify prognostic gangliosides in tissue sections of rat intracranial allografts of rat glioma and mouse intracranial xenografts of human medulloblastoma. In the healthy adult rodent brain, GM1 and GD1 were the main types of glycolipids. Both gangliosides were absent in both intracranial transplants. The ganglioside GM3 was not present in the healthy adult brain but was highly expressed in rat glioma allografts. In combination with tandem mass spectrometry GM3 (d18:1/C24:0) was identified as the most abundant ganglioside species in the glioma allotransplant. By contrast, mouse xenografts of human medulloblastoma were characterized by prominent expression of the ganglioside GM2 (d18:0/C18:0). Together, these data demonstrate that tissue-based MALDI-MSI of gangliosides is able to discriminate between different brain tumors and may be a useful clinical tool for their classification and grading.
The script is well structured, and the dialogues seem natural. There is no attempt to create a feel-good movie. The story is simple and has no twists. It is easy to follow and the execution is smooth with the right direction. The performances are both good and bad, and the script does not discriminate on the basis of class, creed or caste.
We get a glimpse of the life of a student in the campus and the influence of studies is shown in a positive manner. It also shows the sacrifice of a parent in a safe way. However, the students seem a perfect match for the faculty, and it is also shown clearly. They enjoy the luxuries but feel no responsibility towards society. The entire thing looks as if it is part of the movie itself and not a rationalisation of the real life.
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One of the drawbacks to this approach was that we needed to recompile every time we made even a small change to the code. This slowed everything else we were doing down and because we couldn't always have someone waiting for the application to finish building. 827ec27edc